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| 产品名称： | Enterovirus D68 |
|---|---|
| 商品货号： | TS159208 |
| Classification： | Picornaviridae, Enterovirus, Enterovirus D |
| Agent： | Enterovirus D68 |
| Strain： | US/KY/14-18953 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Effect on Host： | CPE, refractile rounding and sloughing |
| Recommended Host： | RD cells (ATCC® CCL-136™) |
| Growth Conditions： | Temperature: 33°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-95% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01-0.001). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 2-3 days |
| Name of Depositor： | Centers for Disease Control and Prevention |
| Source： | Nasopharyngeal swab from patient with respiratory illness, Kentucky, September 2014 |
| References： | Brown BA, et al. Seven Strains of Enterovirus D68 Detected in the United States during the 2014 Severe Respiratory Disease Outbreak. Genome Announc 2(6): e01201-14, 2014. PubMed: 25414503 |