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| 产品名称： | pCD.2 |
|---|---|
| 商品货号： | TS159362 |
| Designations： | pCD.2 |
| Depositors： | PC Zambryski |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli DH5 |
| Vector Information： | Size (kb): 6.2199997901916500 DESCRIPTION OF VECTOR: Intact vector size: 6.220 Type of vector: plasmid Cloning sites: NdeI NcoI AvrII BglII BclI BamHI SmaI SalI PstI Polylinker sites: NdeI NcoI AvrII BglII BclI BamHI SmaI SalI PstI Construction: pRS314 Features (with orientation and position when available): replicon: ColE1, 1-362 coding sequence: bla, replicon: ARS4, 2066-2442 centromere: CEN6, 2028-2053 marker(s): TRP1, ->, 2976-3650 promoter: PADH, 4437-5165 MCS: NdeI..PstI, ->, 5661-5730 ribosome-binding site: GAL4, ->, 5166-5607 transcription terminator: ADC1 transcriptional terminator (TADH), 5748-5938 Vector: pCD.2 (plasmid) Promoters: Promoter PADH Construction: pRS314 Marker(s):crbR Construct size (kb): 6.21999979019165 Features: marker(s): TRP1 promoter: PADH replicon: ARS4 replicon: ColE1 MCS: NdeI..PstI ribosome-binding site: GAL4 transcription terminator: ADC1 transcriptional terminator (TADH) centromere: CEN6 coding sequence: bla |
| Applications： | cloning vector shuttle vector vector component of two-hybrid screening system |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.2; PstI--6.2; HindIII--4.5, 1.7; NdeI--6.2. Useful in two hybrid screening in yeast. A CEN-based plasmid with the Gal4 DNA binding domain. It has lower copy number than 2 micron based plasmids. Contains unique rstriction sites (BglII, BclI and BamHI) compatible with 5 GATC overhangs in all three reading frames. |
| Media： | ATCC® Medium 1637: LB medium (ATCC medium 1065) with 100 mcg/ml carbenicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Durfee T, et al. New tools for protein linkage mapping and general two-hybrid screening. Yeast 15: 1761-1768, 1999. PubMed: 10590464 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |