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| 产品名称： | lambdaSK22 clone 1 |
|---|---|
| 商品货号： | TS159363 |
| Designations： | lambdaSK22 clone 1 |
| Depositors： | ER Zabarovsky, G Winberg, G Klein |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 47.7999992370605500 Vector: lambdaSK22 (phage, lambda - replacement) Marker(s):red+ gam+ Construct size (kb): 47.79999923706055 Features: insert detection: Spi+ marker(s): red+ gam+ replicon: lambda, pMB1, M13 |
| Applications： | vector containing primer sites useful for sequencing vector for constructing genomic libraries vector for other uses vector permitting positive selection for inserts |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--25.0, 13.5, 9.5; EcoRI--25.0, 13.5, 9.5; SalI--20.0, 9.5, 9.2, 4.2, 3.9. Vector permitting positive selection (Spi) of recombinants. Useful for generating jumping and linking libraries and conversion to phagemid (without selection). Permits construction using partial filling-in. The polylinker sites given are those associated with the longer arm. The sites associated with the other arm are: SalI BamHI EcoRI XhoI NotI NaeI SfiI AvrII NheI NcoI XbaI. The restriction sites to be used for conversion to phagemid are: ClaI BspMII SalI. The sizes of the major components are (kb): arms--20.3, 9.2; stuffer fragment--13.7; phagemid segments--3.9 (longer arm), 0.7. The order of the major features in this plasmid is: arm - ClaI BspMII SalI - pMB1 ori - ampR - M13 ori - SacI/MCS/SalI - stuffer - SalI/MCS/XbaI - ClaI SalI - arm. |
| References： | Zabarovsky ER, et al. Lambda SK diphasmids: phage lambda vectors for genomic, jumping, linking and cDNA libraries. Gene 127: 1-14, 1993. PubMed: 8486275 Zabarovsky ER, Winberg GN. Improved methods and new vectors for creating libraries of cloned genes. Sov. Sci. Rev. 11: 1-65, 1992. |
| Shipped： | freeze-dried |