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| 产品名称： | pLS207 981 |
|---|---|
| 商品货号： | TS159396 |
| Designations： | pLS207 981 |
| GenBank Number： | M14340 |
| Species： | Streptococcus pneumoniae (Klein) Chester |
| Depositors： | Brookhaven National Laboratory, SA Lacks, Brookhaven National Laboratory |
| Vector： | Construct size (kb): 9.899999618530273 |
| Insert： | DNA: genomic Insert lengths(kb): 3.5 Gene product: dpnC |
| Insert Size (kb)： | 3.5 |
| Media： | ATCC® Medium 1420: Trypticase soy agar with defibrinated sheep blood, sucrose and 0.5 mcg/ml tetracycline |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains NcoI, XmnI (2), and PstI sites. Strain is noncapsulated and never reverts to encapsulated form. Should be treated as a class II biohazard. A 1.7 kb EcoRV/PstI fragment terminating at the PstI site near the distal end of the insert contains the DpnI-specific gene. The ATG for dpnC begins at nucleotide 227 from the EcoRV site and encodes a 30 kDa protein. The dpnD coding sequence, transcribed in the same orientation as dpnC, extend from approximately 1050 to 1500 bp from the EcoRV site. According to the published sequence, the limits of the genes are as follows: dpnC from 526 to 1041 and dpnD from 1038 to 1499. |
| References： | Lacks SA. Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of Streptococcus pneumoniae. US Patent 4,960,707 dated Oct 2 1990 Lacks SA, et al. Genetic basis of the complementary DpnI and DpnII restriction systems of S. pneumoniae: an intracellular cassette mechanism. Cell 46: 993-1000, 1986. PubMed: 3019562 Sanford A Lacks, personal communication |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |