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| 产品名称： | pMLC3spec |
|---|---|
| 商品货号： | TS159418 |
| Designations： | pMLC3spec |
| GenBank Number： | X05451 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 3.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.07999999821186066 Tissue: fetal muscle Gene product: myosin, light polypeptide 1, alkali; skeletal, fast MYL1 |
| Insert Size (kb)： | 0.080 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.7; BamHI--3.0, PstI--3.0; HindIII--3.0; SmaI--3.0. An EcoRI digest analyzed on a 6% acrylamide gel revealed a 0.08 kb band. This is specific to human myosin light chain 3 mRNA and detects a mRNA of 850 nt in human fast muscle fibers. There is no cross-hybridization to other species. The clone contains 3 inserts of 0.08 kb each. |
| References： | Seidel U, et al. Alkali myosin light chains in man are encoded by a multigene family that includes the adult skeletal muscle, the embryonic or atrial, and nonsarcomeric isoforms. Gene 66: 135-146, 1988. PubMed: 2458299 Seidel U, et al. The complete nucleotide sequences of cDNA clones coding for human myosin light chains 1 and 3. Nucleic Acids Res. 15: 4989, 1987. PubMed: 3601661 Hans H Arnold, personal communication |