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| 产品名称： | Hubs-5B Hs1 |
|---|---|
| 商品货号： | TS159527 |
| Designations： | Hubs-5B Hs1 |
| GenBank Number： | M30773 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 45.5 DESCRIPTION OF VECTOR COMPONENT: Name of vector: lambdagt11 Intact vector size: 43.700 Type of vector: phage Vector end: EcoRI Vector end: EcoRI Cloning sites: EcoRI Polylinker sites: Cloning capacity: 7.2 kb Construction: lambdagt7-lac5(b522 nin5); lambdagt4(cI857 S100 nin5) Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ promoter: lac replicon: lambda repressor gene: cI857 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: PPP3R* Genomic copy number: unique Gene name: protein phosphatase 3 (formerly 2B), regulatory subunit (calcineurin B) Contains complete coding sequence?: Y Chromosome: 2; Localization: 2 Tissue: brainstem Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 1.8 Cross references: DNA Seq. Acc.: M30773 Nucleotides 1-1836 of the insert correspond to nucleotides 713-2548 of M30773. Insert lengths(kb): 1.799999952316284 Tissue: brainstem Gene product: protein phosphatase 3 (formerly 2B), regulatory subunit (calcineurin B) PPP3R* |
| Insert Size (kb)： | 1.800 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--24.0, 20.0, 0.35, 0.28 (doublet); BamHI--16.0, 11.0, 6.5 (doublet), 5.6; BglII--19.0, 15.0, 7.0, 2.4, 0.7; EcoRV--5.6, 5.3, 4.5 (doublet), 3.7 (doublet), 3.3, 3.1, 2.5, 1.5, 1.4 (doublet), 1.3, 0.75, 0.6 (doublet); HindIII--21.0, 9.4, 6.0, 4.4, 3.8, 0.6; NdeI--20.0, 5.8 (doublet), 3.8, 2.4, 2.2, 1.7, 1.6, 0.55. The insert contains the following restriction sites (approximate kb from the 5 end): BglII--0.61; EcoRI--0.27, 0.50, 1.57; EcoRV--0.53, 1.51; HincII--1.12; HindIII--0.13; NdeI--0.87; SphI--1.76. Corresponds to nt 804-2548 of the GenBank record. It does not include the complete coding sequence (nt 759-1271). Detects a 2.8-2.9 kb mRNA in rabbit brain, and a slightly smaller transcript in murine muscle and kidney. |
| References： | Guerini D, et al. Isolation and sequence of a cDNA clone for human calcineurin B, the calcium-binding subunit of the Ca(2+)/calmodulin-stimulated protein phosphatase. DNA 8: 675-682, 1989. PubMed: 2558868 |