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产品简介
购买须知
| 产品名称： | lambdaACT2 - human breast cDNA library |
|---|---|
| 商品货号： | TS159549 |
| Designations： | lambdaACT2 - human breast cDNA library |
| Species： | Homo sapiens, human |
| Depositors： | SJ Elledge |
| Origin： | breast |
| Vector： | Genome: human
Tissue: breast
Type of DNA: cDNA
Insert size: 0.7 – 7 kb
Number of independent recombinants: 1.0 x 108
Non-recombinantsxa0(%): 5.0
Insert 5’ end:
Insert 3’ end: XhoI adapters
Titer: 9.5 x 108 pfu/mL
Vector: lambdaACTII
Intact vector size (kb): 42.300
Type of vector: phage
Features:
xa0xa0xa0xa0Cloning sites: xa0xa0NcoI SfiI SmaI BamHI EcoRI XhoI BglII
xa0xa0xa0xa0Promoter: ADCI
xa0xa0xa0xa0Replicon: pMB1, 2 micron, lambda
xa0xa0xa0xa0Repressor gene: cI857
xa0xa0xa0xa0Terminator: ADC1
Construction: lambdaYES, pACT2 (ATCC#87006)
Vector ends: EcoRI, XhoI
Markers: ampR, LEU2 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | In yeast, the ADH promoter drives expression of a fusion protein consisting of the GAL4 activation domain, a hemagglutinin tag, and the sequences directed by the insert. |
| References： | Durfee T, et al. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 7: 555-569, 1993. PubMed: 8384581 Stephen J Elledge, personal communication |