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| 产品名称： | Human rhinovirus 29 |
|---|---|
| 商品货号： | TS159568 |
| Classification： | Picornaviridae, Enterovirus, Rhinovirus A |
| Agent： | Human rhinovirus 29 |
| Strain： | 5582 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | VR-1809™ has been treated for Mycoplasma orale at the ATCC using diethyl ether.xa0 The original item number was VR-504™. Reciprocal relationship with type 44 reported. |
| Effect on Host： | CPE, cell rounding and cell degeneration with sloughing |
| Recommended Host： | HeLa cells (ATCC® CCL-2™) |
| Growth Conditions： | Temperature: 33°C Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 1-7 days at 33°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | VV Hamparian, RM Conant |
| Source： | Human throat washings |
| References： | Ketler A, et al. Characterization and classification of ECHO 28-rhinovirus-coryzavirus agents. Proc. Soc. Exp. Biol. Med. 110: 821-831, 1962. PubMed: 14455433 Kapikian AZ et al. Rhinoviruses: a numbering system. Nature 213: 761-762, 1967. PubMed: 4291698 |