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| 产品名称： | pLDR21 WM2194 |
|---|---|
| 商品货号： | TS159647 |
| Designations： | pLDR21 WM2194 |
| Depositors： | W Messer |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.6500000953674320 Vector: pLDR21 (plasmid) Promoters: Promoter ptac Construction: pT7-7, pJF118EH Marker(s):ampR Construct size (kb): 3.650000095367432 Features: E. coli promoter cassette: lacIq - ptac cassette marker(s): ampR operator: lac promoter: ptac replicon: pMB1 repressor gene: lacIq |
| Applications： | contains easily purifiable cassette(s) for construction lacIq - ptac promoter cassette |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglI--4.0; ClaI--4.0; XhoI--2.3, 1.65. Helper plasmid with an XhoI promoter cassette containing the gene for lacIq and the ptac promoter. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Diederich L, et al. A versatile plasmid vector system for the regulated expression of genes in Escherichia coli. BioTechniques 16: 916-920, 1994. PubMed: 8068348 |
| Shipped： | freeze-dried |