| 产品名称: | pEFZ31 |
|---|---|
| 商品货号: | TS159656 |
| Designations: | pEFZ31 |
| Species: | Homo sapiens, human |
| Depositors: | RL White, Y Nakamura |
| Applications: | RFLPs observed under normal conditions of hybridization and washing. |
| Vector: | Construct size (kb): 5.599999904632568 |
| Insert: | DNA: genomic Insert lengths(kb): 2.900000095367432 Gene product: DNA Segment, single copy D22S32 Alleles: A1, A2 |
| Insert Size (kb): | 2.900 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: freeze-dried |
| Comments: | Restriction digests of the clone give the following sizes (kb): EcoRI--5.6; EcoRI/HindIII--2.9, 2.7; EcoRI/BamHI--5.4; BamHI--5.4, 0.25; PstI--2.8, 2.8; AccI--uncut. Pattern of deposited DNA matches that of our preparation. Codominant segregation demonstrated in 24 3-generation families. RFLPs observed under normal conditions of hybridization and washing. Enzyme(s) not detecting polymorphism: RsaI, BglII, PstI, PvuII, BamHI, HindIII, EcoRI. |
| References: | Krapcho K, et al. Isolation and mapping of a polymorphic DNA sequence (pEFZ31) on chromosome 22 D22S32. Nucleic Acids Res. 16: 5221, 1988. PubMed: 2898761 Yusuke Nakamura, personal communication |