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| 产品名称： | 5HT1Dbeta |
|---|---|
| 商品货号： | TS159657 |
| Designations： | 5HT1Dbeta |
| GenBank Number： | L09732 |
| Species： | Homo sapiens, human |
| Depositors： | MJ Bienkowski, SA Veldman |
| Applications： | in another host, produces protein 5-hydroxytryptamine (serotonin) receptor 1D beta |
| Vector： | Construct size (kb): 8.710000038146973 DESCRIPTION OF VECTOR COMPONENT: Name of vector: p3CLNeo Intact vector size: 7.440 Type of vector: plasmid Vector end: HindIII Vector end: SalI Cloning sites: HindIII SalI Polylinker sites: HindIII SalI Construction: p3CL, neoR Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): marker(s): ampR, G418 promoter: CMV replicon: pMB1, SV40 terminator: bGH polyadenylation Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: HTR1DB* Gene name: 5-hydroxytryptamine (serotonin) receptor 1D beta Contains complete coding sequence?: Y Tissue: placenta, male Type of DNA: genomic Insert 5 end: HindIII Insert 3 end: SalI Insert size (kb): 1.27 Cross references: DNA Seq. Acc.: L09732 Insert lengths(kb): 1.269999980926514 Tissue: placenta, male Gene product: 5-hydroxytryptamine (serotonin) receptor 1D beta HTR1DB* Target Gene: 5-hydroxytryptamine (serotonin) receptor 1D beta |
| Insert Size (kb)： | 1.270 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglI--3.2, 1.9, 1.8, 1.1, 0.7; HindIII/SalI--7.4, 1.3; PstI--6.5, 1.3, 0.9; PvuII--3.5, 2.3, 1.1, 0.78, 0.58, 0.44; SmaI--5.7, 3.0; EcoRI--6.0, 3.0; EcoRI/XhoI--6.0, 2.35, 0.65. The insert contains the following restriction sites (approximate kb from the 5 end): BglI--0.30; NcoI--0.03; PstI--0.13; PvuII--0.80; SmaI--0.82. Includes 22 bp of 5 untranslated sequence, the complete coding region, and 64 bp of 3 untranslated sequence. The order of the major features of the plasmid is: ampR - EcoRI - SV40 ori - SV40 promoter - XhoI - neoR - SV40 polyadenylation - EcoRI - CMV promoter - HindIII - insert - SalI - bGH polyadenylation - PvuI. |
| References： | Veldman SA, Bienkowski MJ. Cloning and pharmacological characterization of a novel human 5-hydroxytryptamine1D receptor subtype. Mol. Pharmacol. 42: 439-444, 1992. PubMed: 1328844 Sarah A Veldman, personal communication |