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| 产品名称： | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| 商品货号： | TS159679 |
| Classification： | Enterobacteriaceae, Escherichia |
| Strain Designations： | S17-1 |
| Application： | transformation host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -80°C or colder |
| Preceptrol®： | no |
| Genotype： | recA pro hsdR RP4-2-Tc::Mu-Km::Tn7 integrated into the chromosome |
| Type Strain： | no |
| Comments： | Recommended mobilization host for pARO180, pARO190, pARO181, and pARO191 (ATCC 77123-77126, respectively) because it contains chromosomally integrated tra genes. A kanamycin-sensitive strain devoid of the E. coli K-12-specific restriction system allowing efficient uptake of foreign DNA. The properties of streptomycin, trimethoprim, and spectinomycin resistance were verified. RP4-Tc::Mu-Km::Tn7 is integrated into the chromosome. |
| Medium： | ATCC® Medium 2928: M9 + 40 ug/ml Proline + 10 ug/ml Trimetropin |
| Growth Conditions： | Temperature: 37°C Atmosphere: Aerobic |
| Name of Depositor： | D Parke, Department of Biology, Yale University, New Haven, CT |
| Chain of Custody： | ATCC |
| References： | McFarlane GJ, et al. A simplified method for conjugal gene transfer into the filamentous cyanobacterium Anabaena sp. ATCC 27893. J. Microbiol. Methods 6: 301-305, 1987. Parke D. Construction of mobilizable vectors derived from plasmids RP4, pUC18 and pUC19. Gene 93: 135-137, 1990. PubMed: 2227423 Simon R, et al. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria. Bio-Technology 1: 784-791, 1983. |