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| 产品名称： | pCDM8-CD97 CD97 cDNA |
|---|---|
| 商品货号： | TS159708 |
| Designations： | pCDM8-CD97 CD97 cDNA |
| GenBank Number： | X84700 |
| Species： | Homo sapiens, human |
| Depositors： | J Hamann |
| Applications： | in another host, produces protein CD97 antigen |
| Vector： | Construct size (kb): 7.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pCDM8 Intact vector size: 4.356 Type of vector: phagemid Vector end: BstXI Vector end: BstXI Cloning sites: HindIII XbaI XhoI BstXI PstI NotI Polylinker sites: HindIII XbaI XhoI BstXI --stuffer--BstXI XhoI PstI NotI XbaI Construction: CMV, SV40 Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): replicon: pMB1, 1-587 replicon: M13, marker(s): supF, promoter: CMV, ->, 1516-2170 promoter for in vitro transcription: T7, ->, 2171-2189 MCS: HindIII...XbaI, ->, 2198-2655 terminator: SV40 splice site and polyadenylation, ->, 2656-3352 replicon: polyoma, 3353-4188 replicon: SV40, 4189-4352 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CD97 Gene name: CD97 antigen Contains complete coding sequence?: Y Chromosome: 19; Localization: 19 p13.12-13.2 Tissue: peripheral blood mononuclear cells, PMA-stimulated Type of DNA: cDNA Insert end: Insert end: Insert size (kb): 2.914 Cross references: DNA Seq. Acc.: X84700 Nucleotides 1-2914 of the insert correspond to nucleotides 8-2921 of X84700. Insert lengths(kb): 2.914000034332275 Tissue: peripheral blood mononuclear cells, PMA-stimulated Gene product: CD97 antigen CD97 Target Gene: CD97 antigen |
| Insert Size (kb)： | 2.914 |
| Media： | ATCC® Medium 1951: LB medium (ATCC medium 1065) with 50 mcg/ml ampicillin and 15 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): XhoI--4.1, 2.9; SmaI--5.1, 1.9; NcoI--2.7, 1.9, 0.85 (doublet), 0.8. Insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.80, 0.96; EcoRI--2.22; EcoRV--0.79; NcoI--0.06, 2.72; NdeI--2.28; PstI--2.11, 2.65; PvuI--2.07; PvuII--0.48, 0.95; SacI--1.69; SmaI--0.35, 2.26. |
| References： | Hamann J, et al. Expression cloning and chromosomal mapping of the leukocyte activation antigen CD97, a new seven-span transmembrane molecule of the secretin receptor superfamily with an unusual extracellular domain. J. Immunol. 155: 1942-1950, 1995. PubMed: 7636245 |