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| 产品名称： | pSRW25 |
|---|---|
| 商品货号： | TS159713 |
| Designations： | pSRW25 |
| Depositors： | CM Thomas |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 12.6000003814697300 Vector: pSRW25 (plasmid) Promoters: Promoter trp Construction: pRK2501, EcoRI-SphI trpR from pSRW21 Marker(s):kanR,tetR Construct size (kb): 12.60000038146973 Features: marker(s): kanR, tetR promoter: trp replicon: pMB1, RK2 |
| Applications： | expression vector |
| Comments： | Plasmid can replicate in most gram-negative bacteria except Bacteroides. Constructed to provide tight but inducible regulation from the trp promoter on a high copy number plasmid. |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Warne SR, et al. Use of a modified Escherichia coli trpR gene to obtain tight regulation of high-copy-number expression vectors. Gene 46: 103-112, 1986. PubMed: 3026921 Christopher M Thomas, personal communication |
| Shipped： | freeze-dried |