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| 产品名称： | 5D5.11.6 5D5 |
|---|---|
| 商品货号： | TS159746 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue： | lymph node |
| Cell Type： | hybridoma:lymphoblast B lymphocyte; somatic cell hybri |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | Strain:xa0 BALB/c (B cell); BALB/c (myeloma) |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Images： |  |
| Derivation： | Animals were immunized with c-Met-IgG fusion protein (cellular receptor for HGF). Lymph node cells were fused with P3X63Ag8U.1 mouse myeloma cells (ATCC CRL-1597). |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against hepatocyte growth factor (HGF) receptor |
| Cellular Products： | immunoglobulin; monoclonal antibody; against hepatocyte growth factor (HGF) receptor |
| Comments： | Animals were immunized with c-Met-IgG fusion protein (cellular receptor for HGF). Lymph node cells were fused with P3X63Ag8U.1 mouse myeloma cells (ATCC CRL-1597). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml. Interval: Maintain cultures at a cell concentration between 1 X 10(5) and 1 X 10(6) cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with an additional 10% fetal bovine serum and 7.5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype： | IgG1 kappa , IgG1; kappa light chain |
| Name of Depositor： | Genetech, Inc. |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| U.S. Patent Number： | 5,686,292 |
| References： | Schwall RH, Tabor KH. Hepatocyte growth factor receptor antagonist antibodies and uses thereof. US Patent 5,686,292 dated Nov 11 1997 Schwall RH, Tabor KH. Nucleic acids encoding hepatocyte growth factor receptor antagonist antibodies. US Patent 5,646,036 dated Jul 8 1997 Schwall RH, Tabor KH. Hepatocyte growth factor receptor antagonists and uses thereof. US Patent 6,468,529 dated Oct 22 2002 |