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| 产品名称： | pAT3 pAT3c, pATIII-2 |
|---|---|
| 商品货号： | TS159748 |
| Designations： | pAT3 pAT3c, pATIII-2 |
| Depositors： | SH Orkin |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.8999996185302730 Vector: pAT3 (plasmid) Promoters: Promoter unknown promoter Marker(s):ampR Construct size (kb): 5.300000190734863 Features: marker(s): ampR promoter: unknown promoter enhancer: unknown enhancer |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--5.4; EcoRI--5.4; PstI--3.8, 1.6. This clone includes the complete coding sequence, some 5 and 3 sequences. This clone detects genomic EcoRI fragments of 11.0 and 4.1 kb. The polymorphic site is located approximately 100 bp downstream of the 3 end of exon 4. Because of the large sizes of the alleles if genomic DNA is digested with NheI alone, detection of the NheI polymorphism is improved with a NheI/SpeI digest. |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Prochownik EV, et al. Isolation of a cDNA clone for human antithrombin III. J. Biol. Chem. 258: 8389-8394, 1983. PubMed: 6305982 Bock SC, Radziejewska E. A NheI RFLP in the human antithrombin III gene (1q23-q25) (AT3). Nucleic Acids Res. 19: 2519, 1991. PubMed: 1675011 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |