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微信小章| 产品名称： | pHM 2.6 pHM2.6 |
|---|---|
| 商品货号： | TS159774 |
| Designations： | pHM 2.6 pHM2.6 |
| Species： | Homo sapiens, human |
| Depositors： | D Stehelin |
| Applications： | Enzyme(s) not detecting polymorphism: HindIII, BglII, BamHI, SstI, PstI. Tested against 5 unrelated individuals. |
| Vector： | Construct size (kb): 7.099999904632568 |
| Insert： | DNA: genomic Insert lengths(kb): 2.599999904632568 Tissue: fetal liver Gene product: avian myeloblastosis viral (v-myb) oncogene homolog MYB Alleles: A2, A1 |
| Insert Size (kb)： | 2.600 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Insert contains HindIII, SstI, and 2 XbaI sites. Restriction digests of the clone give the following sizes (kb): BamHI--7.1; AvaI--7.1; HindIII--4.5, 2.6; PstI--4.5, 1.8, 0.8; EcoRI--4.5, 2.6. Cross-hybridizes with chicken myb sequences. EcoRI RFLP demonstrated co-dominant segregation in 1 informative family of 9. Allele A1 is amplified in ML3 and COLO 210 cell lines. Enzyme(s) not detecting polymorphism: HindIII, BglII, BamHI, SstI, PstI. Tested against 5 unrelated individuals. |
| References： | Hall JM, et al. Oncogenes and human breast cancer. Am. J. Hum. Genet. 44: 577-584, 1989. PubMed: 2564734 Leprince D, et al. The human DNA locus related to the oncogene myb of avian myeloblastosis virus (AMV): molecular cloning and structural characterization. EMBO J. 2: 1073-1078, 1983. PubMed: 6194989 Radice P, et al. Loss of heterozygosity in human germinal tumors. Cytogenet. Cell Genet. 52: 72-76, 1989. PubMed: 2612217 Dozier C, et al. EcoRI RFLP linked to the human myb gene. Nucleic Acids Res. 14: 1928, 1986. PubMed: 3005993 |