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| 产品名称： | pCW26 |
|---|---|
| 商品货号： | TS159778 |
| Designations： | pCW26 |
| GenBank Number： | M80261 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 4.239999771118164 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.279000043869019 Tissue: placenta Gene product: apurinic/apyrimidinic (abasic) endonuclease APE |
| Insert Size (kb)： | 1.279 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.7, 1.2, 0.9; BamHI--4.8; PstI--2.9, 1.9. Restriction digests of the clone give the following sizes (kb): BglII--4.3; EcoRI--2.9, 1.3; PstI--3.8, 0.5; PvuII--2.5, 1.1, 0.4 (doublet); SacI--3.1, 1.2. The insert contains the following restriction sites (bp from the 5 end): BglII--310; PstI--852; PvuII--97, 436; SacI--168. Contains the complete coding sequence, including 122 nt of 5 untranslated region (with at least one translational stop codon in each reading frame), and 204 nt of 3 untranslated region. The GenBank accession number for the genomic sequence is M99703. This record contains correct information for 4 sites in the cDNA sequence (2 in coding regions, 2 in non-coding regions) that were reported in error. Transcription from the T3 promoter gives the sense strand. |
| References： | Demple B, et al. Cloning and expression of APE, the cDNA encoding the major human apurinic endonuclease: definition of a family of DNA repair enzymes. Proc. Natl. Acad. Sci. USA 88: 11450-11454, 1991. PubMed: 1722334 Bruce Demple, personal communication |