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| 产品名称： | YIp5 pRB12 |
|---|---|
| 商品货号： | TS159794 |
| Designations： | YIp5 pRB12 |
| Depositors： | D Botstein |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.5409998893737790 Vector: YIp5 (plasmid) Construction: pBR322, lambda590-Sc2904 Marker(s):URA3,ampR,tetR Construct size (kb): 5.540999889373779 Features: marker(s): ampR, tetR, URA3 replicon: pMB1 terminator: none enhancer: none |
| Applications： | YI-type (integrating) shuttle vector integrating vector shuttle vector |
| Comments： | YI-type integrating shuttle vector. This was constructed by cloning the 1.1 kb HindIII fragment containing URA3 from lambda590-Sc2904 into the AvaI site of pBR322. The 5 - 3 orientation of URA3 is clockwise; SmaI and AvaI sites in the 3 of URA3 are close to the PvuII site of pBR322. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030 Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863 |
| Related Products： | component of:ATCC 77188 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |