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微信小章| 产品名称： | p482.6 |
|---|---|
| 商品货号： | TS159836 |
| Designations： | p482.6 |
| GenBank Number： | X01179 |
| Species： | Homo sapiens, human |
| Depositors： | RM Lawn |
| Applications： | The 6.2 and 4.8 kb fragments are detected by hybridizing a 1.1 kb EcoRI/SstI fragment to genomic DNA digested with XbaI and KpnI. KpnI is used to distinguish a non-intron 22-sequence migrating at 6.6 kb. |
| Vector： | Construct size (kb): 12.19999980926514 |
| Insert： | DNA: genomic Insert lengths(kb): 9.600000381469727 Gene product: coagulation factor VIIIc, procoagulant component (hemophilia A) F8C Alleles: F3, E1, E1, E2, F1, F2, A1, A2 |
| Insert Size (kb)： | 9.600 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--9.6, 2.6; HindIII--9.6, 2.6; PstI--3.5, 2.6, 2.3, 1.2, 0.8, 0.5, 0.3; BamHI--7.3, 4.6; AccI--5.2, 2.9, 1.4, 1.4, 0.45. The insert is an EcoRI fragment of a portion of intron 22. The polymorphic XbaI site is included in the sequence recognized by the probe. The non-intron 2 band (6.6 kb in a KpnI/XbaI double digest) appears to be more precisely 2 bands at 6.6 and 6.8 kb which are X-chromosomal in origin and may be polymorphic (5.4 + 1.4 and 5.2 + 1.4). The 6.2 and 1.4 kb fragments are detected by hybridizing a 1.6 kb BstXI fragment to genomic DNA digested with XbaI and KpnI. This fragment seems superior in detecting the XbaI polymorphism. The 6.2 and 4.8 kb fragments are detected by hybridizing a 1.1 kb EcoRI/SstI fragment to genomic DNA digested with XbaI and KpnI. KpnI is used to distinguish a non-intron 22-sequence migrating at 6.6 kb. |
| References： | Lillicrap DP, et al. Variation of the non-factor VIII sequences detected by a probe from intron 22 of the factor VIII gene. Blood 75: 139-143, 1990. PubMed: 1967212 Taylor SA, et al. A BstXI polymorphism detected by the factor VIII genomic probe p482.6 (F8C). Nucleic Acids Res. 17: 6426, 1989. PubMed: 2570405 Suthers GK, et al. Physical mapping of new DNA probes near the fragile X mutation (FRAXA) by using a panel of cell lines. Am. J. Hum. Genet. 47: 187-195, 1990. PubMed: 2378346 Janco RL, et al. Improved efficacy of carrier assignment in hemophilia A using intragenic probes. Am. J. Hum. Genet. 39: A95, 1986. Youssoufian H, et al. Characterization of five partial deletions of the factor VIII gene. Proc. Natl. Acad. Sci. USA 84: 3772-3776, 1987. PubMed: 3035554 Wion KL, et al. A new polymorphism in the factor VIII gene for prenatal diagnosis of hemophilia A. Nucleic Acids Res. 14: 4535-4542, 1986. PubMed: 3012474 |