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| 产品名称： | BLUR8 pPD8 |
|---|---|
| 商品货号： | TS159853 |
| Designations： | BLUR8 pPD8 |
| GenBank Number： | J00091 |
| Species： | Homo sapiens, human |
| Depositors： | PL Deininger |
| Vector： | Construct size (kb): 4.639999866485596 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: BamHI Vector end: BamHI Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Genomic copy number: repetitive Gene name: Alu consensus sequence, repetitive Contains complete coding sequence?: U Type of DNA: genomic Insert end: Modification: BamHI linkers Insert end: Modification: BamHI linkers Insert size (kb): 0.275 Cross references: DNA Seq. Acc.: J00091 Insert lengths(kb): 0.2750000059604645 Gene product: Alu consensus sequence, repetitive |
| Insert Size (kb)： | 0.275 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | EcoRI--4.7; HindIII--4.7; BamHI--4.4, 0.27; PvuII--4.7; PstI--4.7. Because of the BamHI linkers (CCGGATCCGG), the size of the excised fragment is 275 bp, with 265 bp representing the Alu repeat. Consists of a head to tail dimer of approximately 130 bp with an insert of 31 bp in the right half as compared to the left. There is a 22% mismatch between BLUR8 and the average Alu family sequence (cf. GenBank accession J00092). |
| References： | Rubin CM, et al. Partial nucleotide sequence of the 300-nucleotide interspersed repeated human DNA sequences. Nature 284: 372-374, 1980. PubMed: 6244506 Deininger PL, et al. Base sequence studies of 300 nucleotide renatured repeated human DNA clones. J. Mol. Biol. 151: 17-33, 1981. PubMed: 6276559 Prescott L Deininger, personal communication |