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| 产品名称： | HJ-1 |
|---|---|
| 商品货号： | TS159939 |
| Designations： | HJ-1 |
| GenBank Number： | M84986 |
| Species： | Homo sapiens, human |
| Depositors： | B ODowd |
| Applications： | A 1.4 kb NcoI/SacI fragment has been subcloned into a mammalian expression vector and used to demonstrate function in kidney cell line 293. |
| Vector： | Construct size (kb): 5.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript Intact vector size: 2.960 Type of vector: phagemid Vector end: SacI Vector end: SacI Cloning sites: Polylinker sites: Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, T3, T7 replicon: pMB1, f1 terminator: none Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: HTR1B Gene name: 5-hydroxytryptamine (serotonin) receptor 1B Contains complete coding sequence?: Y Chromosome: 6; Localization: 6 q13 Type of DNA: genomic Insert end: SacI Insert end: SacI Insert size (kb): 2.0 Cross references: DNA Seq. Acc.: M84986 Nucleotides 1-1912 of the insert correspond to nucleotides 1-1912 of M84986. Insert lengths(kb): 2.0 Gene product: 5-hydroxytryptamine (serotonin) receptor 1B HTR1B |
| Insert Size (kb)： | 2.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglII--4.8; HindIII--4.5, 0.26; NcoI/SalI--4.3, 0.78; SstI--2.85, 2.0; XhoI--4.8. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--1.89; BglI--0.86; BglII--0.09; HindIII--0.12; NcoI--0.59; NotI--0.04; PstI--0.05, 0.16, 0.69; PvuI--0.51; PvuII--0.44, 1.35; SmaI--1.37. A 1.4 kb NcoI/SacI fragment has been subcloned into a mammalian expression vector and used to demonstrate function in kidney cell line 293. Contains an intronless open reading frame of 1170 nt that begins at nt 592 of the sequence. Enzymes not detecting polymorphism: BamHI BglI BglII EcoRI HindIII MspI PstI PvuII RsaI SstI TaqI XbaI. |
| References： | Jin H, et al. Characterization of the human 5-hydroxytryptamine1B receptor. J. Biol. Chem. 267: 5735-5738, 1992. PubMed: 1348246 |