微信小陈
微信小章| 产品名称： | pE194 |
|---|---|
| 商品货号： | TS160005 |
| Designations： | pE194 |
| Depositors： | DM Ellis |
| Other IDs： | Nucleotide (GenBank) : VB0131 pE194 cloning vector. |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.7 Vector: pE194 (plasmid) Promoters: Promoter none Construction: natural isolate Marker(s):eryR Construct size (kb): 3.7 Features: marker(s): eryR promoter: none replicon: pE194 terminator: none enhancer: none |
| Applications： | shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): AccI--3.8; PstI--3.8. Temperature sensitive. For full induction, the plasmid requires a period of induction by subinhibitory concentrations of erythromycin. natural isolate from Staphylococcus aureus confers resistance to macrolide, lincosamide, and streptogramin type B "MLS" antibiotics. |
| Media： | ATCC® Medium 1179: VY medium |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Horinouchi S, Weisblum B. Posttranscriptional modification of mRNA conformation: mechanism that regulates erythromycin-induced resistance. Proc. Natl. Acad. Sci. USA 77: 7079-7083, 1980. PubMed: 6938954 Iordanescu S. Relationships between cotransducible plasmids in Staphylococcus aureus. J. Bacteriol. 129: 71-75, 1977. PubMed: 830649 Weisblum B, et al. Plasmid copy number control: isolation and characterization of high-copy-number mutants of plasmid pE194. J. Bacteriol. 137: 635-643, 1979. PubMed: 104975 Horinouchi S, Weisblum B. Nucleotide sequence and functional map of pE194, a plasmid that specifies inducible resistance to macrolide, lincosamide and streptogramin type B antibiotics. J. Bacteriol. 150: 804-814, 1982. PubMed: 6279574 |
| Shipped： | frozen |