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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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HPV-6b plasmid in E. coli

货号 TS160017
中文名称 null
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产品简介
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产品名称: HPV-6b plasmid in E. coli
商品货号: TS160017
Designations: HPV-6b plasmid in E. coli
GenBank Number:

X00203

Species: human papilloma virus type 6b
Depositors: E de Villiers, H zur Hausen
Origin:
condyloma acuminatum
Vector:

pUC19 (phagemid)
Size (kb): 2.686
Construction: pUC71K
Marker(s): ampR (50 ug/ml)
Features: Insert detection: lacZ; Promoter: lac; Replicon: pMB1; MCS:
EcoRI...HindIII
Sequencing primers: M13(-21), M13 reverse

Construct size (kb): 10.6
Insert site: BamHI
Excise insert: BamHI

Insert:
Gene product: human papilloma virus type 6b genome
Insert size (kb): 7.9
Insert ends: BamHI
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Related Products:
Purified plasmid DNA: TS160017
Biosafety Level: 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments:
Full-length genome of pHPV-6b cloned into BamH1 site of pBR322.
Constructed by linearizing the virus at the unique BamHI site (nt 4722) in the coding region for L2.
The depositor has requested that this deposit be distributed exclusively for scientific and not for commercial purposes.
Restriction digests of the clone give the following sizes (kb): BamHI - 8.0, 2.7; BglI - 7.4, 2.2, 1.0. - ATCC staff
Classification: Papovavirus, Papillomavirus, recombinant
References:

Schwarz E, et al. DNA sequence and genome organization of genital human papillomavirus type 6b. EMBO J. 2: 2341-2348, 1983. PubMed: 6321162

Harald zur Hausen, personal communication