微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pIC-ER-N pIC 20 ER S8 |
|---|---|
| 商品货号： | TS160036 |
| Designations： | pIC-ER-N pIC 20 ER S8 |
| GenBank Number： | M12674 |
| Species： | Homo sapiens, human |
| Depositors： | H Ahrens |
| Applications： | produces protein estrogen receptor |
| Vector： | Construct size (kb): 3.5 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pIC19R Intact vector size: 2.695 Type of vector: plasmid Vector end: BamHI Vector end: BamHI Cloning sites: EcoRI ClaI EcoRV XbaI BglII XhoI SacI NruI HindIII PstI SalI BamHI SmaI NarI Polylinker sites: EcoRI ClaI EcoRV XbaI BglII XhoI SacI NruI HindIII PstI SalI BamHI SmaI EcoRI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): ampR replicon: pMB1 Cross references: DNA Seq. Acc.: L08911 |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: ESR Genomic copy number: unique Gene name: estrogen receptor Contains complete coding sequence?: U Chromosome: 6; Localization: 6 q25.1 Type of DNA: cDNA Insert end: Sau3AI Insert end: Sau3AI Insert size (kb): 0.8 Cross references: DNA Seq. Acc.: M12674 Insert lengths(kb): 0.800000011920929 Gene product: estrogen receptor ESR Alleles: A1, A2 Target Gene: estrogen receptor |
| Insert Size (kb)： | 0.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.7; BglI--1.85, 1.2, 0.60; HindIII--3.7; PstI--3.3, 0.33; PvuII--2.4, 0.66, 0.50; EcoRI--2.7, 0.9. The insert contains the following restriction sites (approximate kb from the 5 end): BglI--0.15; HindIII--0.69; PstI--0.04; PvuII--0.02; StuI--0.29, 0.39. Overproduces the fusion protein in E. coli, the majority of which is found in inclusion bodies. It binds DNA. Contains the coding sequence for amino acids 109-374. The recombinant fusion protein contains an additional 23 N-terminal amino acids and additional C-terminal amino acids. |
| References： | Ahrens H, et al. Overproduction of full-length and truncated human estrogen receptors in Escherichia coli. Receptor 2: 77-92, 1992. PubMed: 1472947 |