宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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alphaRI12

货号 TS160040
中文名称 null
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产品简介
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产品名称: alphaRI12
商品货号: TS160040
Designations: alphaRI12
Species: Homo sapiens, human
Depositors: YB Yurov
Vector: Construct size (kb): 6.45
Name of vector: pBR325
Intact vector size: 5.996
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: AatII AsuII AvaI ClaI HgiEII HindIII Tth111I BamHI EcoRI EcoRV (MstI-Cm?) NcoI PstI PvuI SalI SphI XmaIII XmnI
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, cmlR, tetR
replicon: pMB1
Insert: Genome: Homo sapiens
Gene symbol: D6Z1
Genomic copy number: repetitive
Gene name: DNA Segment, repetitive
Contains complete coding sequence?: U
Chromosome: 6; Localization: 6 cen
Type of DNA: genomic
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 0.34
Genome: Homo sapiens
Gene symbol: D6Z1
Type of nucleic acid: genomic
Restriction enzyme: TaqI
Restriction enzyme: BamHI
Insert Size (kb): 0.340
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--6.3, 0.3; PstI--6.6; SalI--6.7; BglI--2.6, 2.4, 1.2 + smaller; PvuII--3.8, 2.8.
Similar to p308.
References:

Yurov YB, et al. Collection of alpha-satellite DNA probes: highly polymorphic markers for centromeric regions of all human chromosomes. Cytogenet. Cell Genet. 51: 1114, 1989.

Yu B Yurov, personal communication