| 产品名称: | alphaRI12 |
|---|---|
| 商品货号: | TS160040 |
| Designations: | alphaRI12 |
| Species: | Homo sapiens, human |
| Depositors: | YB Yurov |
| Vector: | Construct size (kb): 6.45 Name of vector: pBR325 Intact vector size: 5.996 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: AatII AsuII AvaI ClaI HgiEII HindIII Tth111I BamHI EcoRI EcoRV (MstI-Cm?) NcoI PstI PvuI SalI SphI XmaIII XmnI Polylinker sites: Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR, cmlR, tetR replicon: pMB1 |
| Insert: | Genome: Homo sapiens Gene symbol: D6Z1 Genomic copy number: repetitive Gene name: DNA Segment, repetitive Contains complete coding sequence?: U Chromosome: 6; Localization: 6 cen Type of DNA: genomic Insert end: EcoRI Insert end: EcoRI Insert size (kb): 0.34 Genome: Homo sapiens Gene symbol: D6Z1 Type of nucleic acid: genomic Restriction enzyme: TaqI Restriction enzyme: BamHI |
| Insert Size (kb): | 0.340 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments: | Restriction digests of the clone give the following sizes (kb): EcoRI--6.3, 0.3; PstI--6.6; SalI--6.7; BglI--2.6, 2.4, 1.2 + smaller; PvuII--3.8, 2.8. Similar to p308. |
| References: | Yurov YB, et al. Collection of alpha-satellite DNA probes: highly polymorphic markers for centromeric regions of all human chromosomes. Cytogenet. Cell Genet. 51: 1114, 1989. Yu B Yurov, personal communication |