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| 产品名称： | HFBDV22 |
|---|---|
| 商品货号： | TS160060 |
| Designations： | HFBDV22 |
| GenBank Number： | T06562 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 4.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: TLE3* Gene name: transducin-like enhancer protein (enhancer of split) Contains complete coding sequence?: N Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Type of DNA: cDNA Insert end: EcoRI Insert end: EcoRI Insert size (kb): 0.8 Cross references: DNA Seq. Acc.: M99438; EST: EST04451 Nucleotides 1-335 of the insert correspond to nucleotides 2007-2341 of M99438 with 97% identity. DNA Seq. Acc.: T06562; EST: EST04451; Primer: M13-21; THC id: THC117710 DNA Seq. Acc.: T06563; EST: EST04452; Primer: M13 Reverse Insert lengths(kb): 0.800000011920929 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: transducin-like enhancer protein (enhancer of split)( transducin-like enhancer of split 3, transducin-like enhancer 3) TLE3* |
| Insert Size (kb)： | 0.800 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--4.0; EcoRI--3.2, 0.8. Verified to contain EST04451 by single-pass sequencing. Based on the size of the insert and the alignment with GenBank accession M99438 (2357 nt, CDS 22-2340), this clone appears to contain part of the coding region and the 3 untranslated region. EST04452 does not match a nucleotide sequence record. |
| References： | Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434 |