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微信小章| 产品名称： | AFT024 IRR |
|---|---|
| 商品货号： | TS160127 |
| Organism： | Mus musculus, mouse |
| Tissue： | Liver; stroma |
| Cell Type： | Fibroblast SV40 immortalized, SV40 transformed |
| Product Format： | frozen |
| Morphology： | Fibroblast |
| Culture Properties： | Adherent |
| Biosafety Level： | 2 xa0Cells contain SV-40 Viral DNA sequences Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS Publication No. (CDC) 93-8395. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office (2007). The entire text is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm. Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 14 to 14.5 day gestation embryo |
| Applications： | These cells are provided for use as feeder cells to support the growth of purified mouse and human CD34+CD38- hematopoietic stem/progenitor cells. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Comments： | These cells have been growth-arrested by irradiation with 12,000 rads.xa0 The cells will begin to deteriorate 2 weeks after plating and may no longer support the growth of cells. We recommend that you do not keep the cells in culture for longer than 2 weeks. It is recommended that the feeder cells be plated 24 hours before use at 5 to 6 X 106 cells/T75 in order to obtain a 100% confluent monolayer for stem cells growth. Once the feeder cells have attached, the culture medium can be changed to accommodate the cells to be supported. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Medium Renewal: twice a week or when pH decreases |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor： | ATCC |
| Deposited As： | Mus musculus |
| References： | Moore KA, et al. Hematopoietic activity of a stromal cell transmembrane protein containing epidermal growth factor-like repeat motifs. Proc. Natl. Acad. Sci. USA 94: 4011-4016, 1997. PubMed: 9108096 Miller JS, et al. Single adult human CD34(+)/Lin-/CD38(-) progenitors give rise to natural killer cells, B-lineage cells, dendritic cells, and myeloid cells. Blood 93: 96-106, 1999. PubMed: 9864151 Moore KA, et al. In vitro maintenance of highly purified, transplantable hematopoietic stem cells. Blood 89: 4337-4347, 1997. PubMed: 9192756 Hachney JA, et al. A molecular profile of a hematopoietic stem cell niche. Proc. Natl. Acad. Sci. USA 99: 13061-13066, 2002. PubMed: 12226475 Punzel M, et al. The myeloid-lymphoid initiating cell (ML-IC) assay assesses the fate of multipotent human progenitors in vitro. Blood 93: 3750-3756, 1999. PubMed: 10339481 Thiemann FT, et al. The murine stromal cell line AFT024 acts specifically on human CD34+CD38- progenitors to maintain primitive function and immunophenotype in vitro. Exp. Hematol. 26: 612-619, 1998. PubMed: 9657136 Lewis ID, et al. Umbilical cord blood cells capable of engrafting in primary, secondary, and tertiary xenogeneic hosts are preserved after ex vivo culture in a noncontact system. Blood 97: 3441-3449, 2001. PubMed: 11369635 Hay RJ, Caputo JL, Macy, ML, Eds. (1992) ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo JL. Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988 Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |