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| 产品名称： | ACR-1 |
|---|---|
| 商品货号： | TS160169 |
| Designations： | ACR-1 |
| Applications： | ACR-1 is free of ribosomal gene sequences and is specific for the conserved region immediately distal to the rRNA genes on all 5 acrocentric chromosomes. |
| Vector： | Construct size (kb): 4.300000190734863 |
| Insert： | DNA: genomic Insert lengths(kb): 1.600000023841858 Gene product: DNA Segment, numerous copies DNF20S1 |
| Insert Size (kb)： | 1.600 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--2.7, 1.6; EcoRI--2.8, 1.5; HindIII--4.3; BamHI--4.3; SacI--4.3. The insert contains an EcoRI site. This is an acrocentric chromosome-specific clone that recognizes a "unique" sequence distal to the ribosome gene cluster on the short arm of all 5 acrocentric chromosomes. It is a subclone of DJ15, a 15 kb BamHI bacteriophage clone containing ribosomal RNA gene sequences, isolated as a junction fragment from the 5 end of the ribosomal gene tandem repeat cluster. Because DJ15 was derived from a total human genomic library, the insert could have been derived from any one of the 5 acrocentrics. ACR-1 is free of ribosomal gene sequences and is specific for the conserved region immediately distal to the rRNA genes on all 5 acrocentric chromosomes. The insert does not hybridize to mouse DNA. |
| References： | Worton RG, et al. Human ribosomal RNA genes: orientation of the tandem array and conservation of the 5 end. Science 239: 64-68, 1988. PubMed: 3336775 |