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| 产品名称： | pCaD4 |
|---|---|
| 商品货号： | TS160175 |
| Designations： | pCaD4 |
| Species： | Cauliflower mosaic virus |
| Depositors： | JE Schoelz |
| Vector： | Construct size (kb): 12.39400005340576 |
| Insert： | DNA: genomic Insert lengths(kb): 8.0 |
| Insert Size (kb)： | 8.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The clone contains the following restriction sites (using the viral numbering scheme from 1 to the SalI site at 4833): BamHI--1926, 2148, 8921; EcoRI--408, 2414, 2474, 8546, 10009, 10681; SacI--3750, 10185; PvuII--4000, 6249, 10681; SalI--4833, 9196. Restriction digests of the clone give the following sizes (kb): SalI--8.2, 4.4; EcoRI--6.5, 2.5, 2.2, 1.5, 0.5; HindIII--4.3, 4.0, 1.2, 1.0, 0.6 (doublet), 0.5; BamHI--6.8, 6.0; PstI--6.5, 4.4, 2.2. Known genes have these approximate positions (virus numbering scheme): I: 300-1300; II (aphid helper component): 1300-1800; III: 1800-2200; IV (coat protein):2200-3600; V (reverse transcriptase): 3600-5600; VI (inclusion body protein): 5800-7300. This strain was isolated from crucifers in Davis, California. This was constructed by linearizing the full-length CaMV DNA with SalI and inserting it into the SalI site of pBR322. |
| Classification： | Caulimovirus |
| References： | Gardner RC, et al. The complete nucleotide sequence of an infectious clone of cauliflower mosaic virus of M13mp7 shotgun sequencing. Nucleic Acids Res. 9: 2871-2888, 1981. PubMed: 6269062 Schoelz JE, et al. Properties of an unusual strain of cauliflower mosaic virus. Phytopathology 76: 451-454, 1986. James E Schoelz, personal communication |