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微信小章| 产品名称： | Orientia chuto |
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| 商品货号： | TS160196 |
| Classification： | Rickettsiaceae |
| Agent： | Orientia chuto |
| Strain： | Dubai |
| Biosafety Level： | 3
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | Vapor phase of liquid nitrogen |
| Images： |  |
| Comments： | New Taxon. It is recommended to determine viability by a PCR-based assay as agent does not produce cytopathic effects. |
| Effect on Host： | Toxicity observed |
| Recommended Host： |
Vero cells (ATCC® CCL-81™)
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| Growth Conditions： | Temperature: 35°C Recommendations for Infection: Plate cells 24-36 hours prior to infection and infect when cultures are 70-90% confluent. Remove medium and inoculate with a small volume of inoculum (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI. Centrifuge at 800 x g at room temperature for 75 minutes - 2 hours. End adsorption by adding growth medium. Incubation: 15 days |
| Name of Depositor： | L Izzard, Australian Rickettsial Reference Laboratory |
| Source： | Clinical female patient with acute scrub typhus infection, Dubai, United Arab Emirates, 2006. |
| References： | Izzard L, et. al. Isolation of a novel Orientia species (O. chuto sp. nov.) from a patient infected in Dubai. J. Clin. Microbiol. 48(12): 4404-4409, 2010. PubMed: 20926708 |