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| 产品名称： | pDM1 |
|---|---|
| 商品货号： | TS160432 |
| Designations： | pDM1 |
| Depositors： | DA Mead |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.7999999523162840 Vector: pDM1 (plasmid) Construction: bacteriophage f1, pAT153 Marker(s):ampR Construct size (kb): 3.799999952316284 Features: marker(s): ampR replicon: pMB1 |
| Applications： | contains easily purifiable cassette(s) for construction vector permitting production of single-stranded DNA |
| Comments： | Contains an easily isolated f1 intergenic region for conversion of any plasmid to an ssDNA vector (use SalI + AccI + HincII). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mead DA, et al. Single stranded DNA SP6 promoter plasmids for engineering mutant RNAs and proteins: synthesis of a stretched preproparathyroid hormone. Nucleic Acids Res. 13: 1103-1118, 1985. PubMed: 3858795 |
| Shipped： | freeze-dried |