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| 产品名称： | pBB2T1 16C8n |
|---|---|
| 商品货号： | TS160465 |
| Designations： | pBB2T1 16C8n |
| Species： | Mus musculus, mouse |
| Depositors： | ET Cocuzzi, DT Denhardt |
| Applications： | in another host, produces protein tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor) |
| Vector： | Construct size (kb): 10.97000026702881 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.6949999928474426 Tissue: fibroblast, embryo Gene product: tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor) Timp Target Gene: tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor) |
| Insert Size (kb)： | 0.695 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): NheI--10.0, 0.7; EcoRV--6.2, 2.2, 1.4, 0.4; PstI--11.0; KpnI--11.0; EcoRI--11.0. Insert contains the following restriction sites (approximate kb from the 5 end): EcoRV--0.26; PstI--0.39. Cotransfection of Sf9 insect cells with the clone and the linearized wild-type Autographa californica nuclear polyhedrosis virus (AcMNPV) (ATCC VR-1345) allows production of a recombinant virus expressing Timp and beta-galactosidase. Transfected insect cells should be grown at 27C. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Cocuzzi ET, et al. Expression and secretion of active mouse TIMP-1 using a baculovirus expression vector. Inflammation 18: 35-43, 1994. PubMed: 8206645 |