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| 产品名称： | lambdaSK23 clone 1 |
|---|---|
| 商品货号： | TS160493 |
| Designations： | lambdaSK23 clone 1 |
| Depositors： | ER Zabarovsky, G Winberg, G Klein |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 47.7999992370605500 Vector: lambdaSK23 (phage, lambda - replacement) Marker(s):red+ gam+ Construct size (kb): 47.79999923706055 Features: insert detection: Spi+ marker(s): red+ gam+ replicon: lambda, pMB1, M13 |
| Applications： | vector containing primer sites useful for sequencing vector for constructing genomic libraries vector for other uses vector permitting RNA synthesis in vitro vector permitting positive selection for inserts |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--23.0, 14.0, 11.0; SalI--21.0, 11.0, 9.0, 6.6; XhoI--23.0, 14.0, 11.0. Vector permitting positive selection (Spi) of recombinants. Useful for generating jumping and linking libraries and conversion to phagemid (with selection). Permits construction using partial filling-in. The restriction sites to be used for conversion to phagemid are: SalI ApaI RsrII NheI. Cloning capacity: 18.9 kb. The sizes of the major components are (kb): arms--20.3, 9.2; stuffer fragment--13.7; phagemid segments--2.6 (longer arm), 1.9. The order of the major features in this plasmid is: arm - SalI/NheI - ampR - M13 ori - SacII/MCS/NaeI - stuffer - NaeI/MCS/XbaI - pMB1 ori - NheI/SalI - arm. |
| References： | Zabarovsky ER, et al. Lambda SK diphasmids: phage lambda vectors for genomic, jumping, linking and cDNA libraries. Gene 127: 1-14, 1993. PubMed: 8486275 Zabarovsky ER, Winberg GN. Improved methods and new vectors for creating libraries of cloned genes. Sov. Sci. Rev. 11: 1-65, 1992. |
| Shipped： | freeze-dried |