Construct size (kb): 6.5

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pCR3
Intact vector size: 5.100
Type of vector: phagemid
Vector end: TA site
Vector end: TA site
Cloning sites: TA cloning between EcoRI sites
Polylinker sites: HindIII KpnI SacI BamHI SpeI XmaIII BstXI EcoRI EcoRI PstI
EcoRV BstXI NotI XhoI XbaI ApaI
Construction: pcDNA3
Features (with orientation and position when available):
promoter: T7, ->
MCS: HindIII/.../TA cloning site/.../ApaI, ->
promoter: SP6, other: bovine growth hormone polyA signal, ->
replicon: pMB1
marker(s): kanR, promoter: SV40, replicon: SV40, marker(s): ampR, replicon: f1, ->
promoter: cytomegalovirus, ->
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: NKAT2*
Gene name: natural killer associated transcript 2
Contains complete coding sequence?: Y
Chromosome: 19; Localization: 19
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 1.423
Cross references: DNA Seq. Acc.: L41268
Nucleotides 1-1423 of the insert correspond to
nucleotides 27-1449 of L41268.

Insert lengths(kb): 1.422999978065491

Gene product: natural killer associated transcript 2 NKAT2*

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): BamHI--7.0; EcoRI--5.6, 1.4; NotI--7.0; SacI--4.9, 1.1, 0.5, 0.084; XhoI--5.6, 0.82.

The insert contains the following restriction sites (approximate kb from the 5 end): SacI--0.49; XhoI--0.67.