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| 产品名称： | pMDY1 phagemid in E. coli |
|---|---|
| 商品货号： | TS160649 |
| Designations： | pMDY1 phagemid in E. coli |
| GenBank Number： | L00727 |
| Depositors： | CS Richards |
| Applications： | Contains sequence useful for DNA diagnostics dystrophia myotonica dystrophia myotonica (includes dystrophia myotonia protein kinase), dystrophia myotonica 1
Detects triplet repeat expansions dystrophia myotonica dystrophia myotonica (includes dystrophia myotonia protein kinase), dystrophia myotonica 1 |
| Vector： | Total size (kb): 4.335 Excise insert: BamHI Vector:xa0pBluescript Type of vector: Phagemid Vector size (kb): 2.96 Insert site(s): BamHI Markers: ampR |
| Insert： | Insert size (kb): 1.377
Source: HumanDNA: genomic Gene symbol: DM Gene name: dystrophia myotonica Genbank accession: L00727 (see above) Contains complete coding sequencexa0?: N Chromosome location: 19 q13.3 Insert ends: BamHI |
| Insert Size (kb)： | 1.377 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | In Escherichia coli containing the plasmid |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--2.9, 1.4; HindIII--4.4; SmaI--3.8, 0.46. The insert contains the following restriction sites (approximate kb from the 5 end): PstI--0.01, 1.20; NarI--0.85; SmaI--0.93. The GCT triplet expansion characteristic of myotonic muscular dystrophy can also be detected using this probe with BanI- or TaqI-digested genomic DNA. |
| References： | Fu YH, et al. An unstable triplet repeat in a gene related to Myotonic Muscular Dystrophy. Science 255: 1256-1258, 1992. PubMed: 1546326 |