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| 产品名称： | c9171 9171 |
|---|---|
| 商品货号： | TS160692 |
| Designations： | c9171 9171 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | MV Olson, L Riles |
| Vector： | Construct size (kb): 0.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pHC79 Intact vector size: 6.430 Type of vector: cosmid Vector end: BamHI Vector end: BamHI Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI Polylinker sites: Construction: pBR322, Charon 4A Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR, tetR replicon: pMB1 terminator: none Cross references: DNA Seq. Acc.: L08873 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Saccharomyces cerevisiae Gene symbol: HmL Gene name: mating-type cassette Contains complete coding sequence?: U Chromosome: III; Localization: III L, 61 cM from CEN Strain: AB972 (ATCC 76269) Type of DNA: genomic Insert end: MboI Insert end: MboI Insert size (kb): Cross references: Gene product: mating-type cassette HML |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Related Products： | component of:ATCC 77284 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 4.542, 0.441, 2.551, 2.208, 3.547, 1.879, 0.553, 0.495, 0.916, 10.083, 2.281, 1.471, 0.769, 1.213, 1.139. Confirmed to contain EcoRI/HindIII fragments of the following sizes (kb): 0.35, 0.46, 0.51, 0.74, 0.89, 1.1, 1.2, 1.5, 1.9, 2.3, 2.7, 3.8, 4.9, ***. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Overlaps clones with ATCC number(s): 70051, 70739. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151 |