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| 产品名称： | GTpZapBglII |
|---|---|
| 商品货号： | TS160729 |
| Designations： | GTpZapBglII |
| GenBank Number： | M34353 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 6.599999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 3.619999885559082 Tissue: glioblastoma SW-1088 cell line Gene product: avian UR2 sarcoma virus oncogene (v-ros) homolog ROS Alleles: A1, A2, A1, A2 |
| Insert Size (kb)： | 3.620 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | The insert includes the following restriction sites (nt positions according to the published sequence): BglII--4382; EcoRI--4623, 6183, 6649, 6845, 6965; HindIII--5438, 6939; BamHI--5631; PstI--5640; BglI--6333; AccI--6376, 6409. Restriction digests of the clone give the following sizes (kb): EcoRI--4.3, 1.5, 0.5, 0.3: BamHI--2.9, 2.3, 1.3; HindIII--3.3, 1.5, 1.1, 0.6; PstI--5.2, 1.3; NotI--3.6, 2.9. Extends from the BglII site at nt 4382 to approximately nt 8000. The open reading frame extends from nt 207 to nt 7247. There is approximately 1 kb of 3 untranslated sequence which has not been fully characterized. Subcloned from lambdagt10.165 which was constructed by adding NotI linkers to a blunt-ended BglII fragment. |
| References： | Birchmeier C, et al. Characterization of ROS1 cDNA from a human glioblastoma cell line. Proc. Natl. Acad. Sci. USA 87: 4799-4802, 1990. PubMed: 2352949 Michael H Wigler, personal communication |