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| 产品名称： | pJRtac99 |
|---|---|
| 商品货号： | TS160757 |
| Designations： | pJRtac99 |
| Depositors： | J Robben, G Volckaert |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 1.3920000791549680 Vector: pJRtac99 (plasmid) Promoters: Promoter tac Construction: pGV451, tac, Tn9 cat Marker(s):cmlR Construct size (kb): 1.392000079154968 Features: marker(s): cmlR promoter: tac replicon: HyRep2 |
| Applications： | vector permitting construction of fusion proteins |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--1.3; HindIII--1.3; BglII--1.3. The vector contains the following restriction sites (approximate kb from nt 1): BglII--0.79; ClaI--0.11; EcoRI--0.33; HindIII--0.02; NcoI--0.12; PstI--0.75; PvuII--0.23. Replication depends on read-through from the tac promoter. Replication may be negatively affected by cloning of large inserts or sequences containing a transcriptional terminator. Expression vector permitting production of heterologous peptides fused to active chloramphenicol acetyltransferase. Repression of this high copy number plasmid is hard to achieve, even using hosts overproducing lacIq. Escherichia coli WK6 is recommended for inducible expression. The replicon is derived from ColE1 and does not contain the RNAII promoter nor most of the antisense RNAI control element coding sequence. This allows compatibility with other ColE1 plasmids. The order of the major features of the plasmid is: HindIII - tac promoter - NcoI - cat/ScaI/MCS/BglII - HyRep2 ori. |
| Media： | ATCC® Medium 2272: LB Medium (Miller) with chloramphenicol 25 mcg/ml |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Robben J, et al. An Escherichia coli plasmid vector system for high-level production and purification of heterologous peptides fused to active chloramphenicol acetyltransferase. Gene 126: 109-113, 1993. PubMed: 7682530 Dekeyzer N, et al. Cloning, expression and purification of a sarcoplasmic calcium-binding protein from the sandworm Nereis diversicolor via a fusion product with chloramphenicol acetyltransferase. Protein Eng. 7: 125-130, 1994. PubMed: 8140089 |
| Shipped： | freeze-dried |