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| 产品名称： | GST-Cip1 |
|---|---|
| 商品货号： | TS160788 |
| Designations： | GST-Cip1 |
| GenBank Number： | L25610 |
| Species： | Homo sapiens, human |
| Depositors： | SJ Elledge |
| Applications： | produces protein cyclin-dependent kinase inhibitor 1A (p21, Cip1) melanoma differentiation associated protein 6, cyclin-dependent kinase inhibitor (p21), wild type p53 activated fragment 1 (Cdk-interacting protein) |
| Vector： | Construct size (kb): 6.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGEX-2TKcs Intact vector size: 5.000 Type of vector: plasmid Vector end: XhoI Vector end: EcoRI Cloning sites: NdeI NcoI XhoI BamHI EcoRI Polylinker sites: NdeI NcoI XhoI BamHI EcoRI Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR promoter: tac replicon: pMB1 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CDKN1A Other gene symbols: CIP1; WAF1* Gene name: cyclin-dependent kinase inhibitor 1A (p21, Cip1) Contains complete coding sequence?: Y Chromosome: 6; Localization: 6 p21.2 Tissue: B lymphocyte (EBV transformed) Type of DNA: cDNA Insert 5 end: XhoI Insert 3 end: EcoRI Insert size (kb): 1.03 Cross references: DNA Seq. Acc.: L25610 Insert lengths(kb): 1.029999971389771 Tissue: B lymphocyte (EBV transformed) Gene product: cyclin-dependent kinase inhibitor 1A (p21, Cip1)( melanoma differentiation associated protein 6, cyclin-dependent kinase inhibitor (p21), wild type p53 activated fragment 1 (Cdk-interacting protein)) CIP1 Target Gene: cyclin-dependent kinase inhibitor 1A (p21, Cip1) |
| Insert Size (kb)： | 1.030 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglI--2.6, 1.5, 1.3, 0.55; EcoRI/XhoI--5.0, 1.1; KpnI--6.0; PstI/XbaI--3.4, 1.4, 1.15; PvuII--4.4, 1.5, 0.3. The insert contains the following restriction sites (approximate kb from the 5 end): BglI--0.15, 0.62; KpnI--0.94. Contains the complete coding sequence (nt 79-573 of the sequence record). Met-1 of the product is linked to GST through the linker sequence SHMPMARGHEGPEVSSLWSRSWARIRRGTEALRGGA. |
| References： | Harper JW, et al. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 75: 805-816, 1993. PubMed: 8242751 Stephen J Elledge, personal communication |