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| 产品名称： | pPD110 |
|---|---|
| 商品货号： | TS160790 |
| Designations： | pPD110 |
| Depositors： | E Bremer |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.5299999713897710 Vector: pPD110 (plasmid) Promoters: Promoter T7 gene 10 Construction: pPD100, pTrc97A Marker(s):cmlR Construct size (kb): 3.529999971389771 Features: marker(s): cmlR promoter: T7 gene 10 replicon: pSC101 MCS: NcoI...HindIII ribosome-binding site: lacZ |
| Applications： | expression vector vector with low copy number |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.5; HindIII--3.5; NcoI--2.7, 0.8. pPD110 (TS160790), pPD111 (ATCC 87225) and pPD112 (ATCC 87226) allow expression of inserts lacking transcriptional and translational initiation signals. These three vectors differ only in the reading frame of the ATG start codon. Deposited for research purposes only. Before any commercial use, the depositor must be contacted. A low copy number plasmid using the T7 gene 10 promoter for selective, overexpression of cloned genes in E. coli. |
| Media： | ATCC® Medium 2272: LB Medium (Miller) with chloramphenicol 25 mcg/ml |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Dersch P, et al. Low-copy-number T7 vectors for selective gene expression and efficient protein overproduction in Escherichia coli. FEMS Microbiol. Lett. 123: 19-26, 1994. PubMed: 7988888 Erhard Bremer, personal communication |
| Shipped： | freeze-dried |