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| 产品名称： | pHEGO-Hyg |
|---|---|
| 商品货号： | TS160852 |
| Designations： | pHEGO-Hyg |
| GenBank Number： | M12674 |
| Species： | Homo sapiens, human |
| Applications： | in another host, produces protein estrogen receptor |
| Vector： | Construct size (kb): 7.400000095367432 DESCRIPTION OF VECTOR COMPONENT: Name of vector: p636 Intact vector size: 5.500 Type of vector: plasmid Vector end: EcoRV Vector end: BamHI Cloning sites: EcoRV, BamHI Polylinker sites: Construction: pHyg, CMV promoter, TK promoter/hygR Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): marker(s): ampR, hygR promoter: CMV replicon: pMB1 terminator: SV40 polyadenylation Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: ESR Genomic copy number: unique Gene name: estrogen receptor Contains complete coding sequence?: Y Chromosome: 6; Localization: 6 q25.1 Tissue: breast cancer cell line, MCF-7 Type of DNA: cDNA Insert 5 end: EcoRV Insert 3 end: BamHI Insert size (kb): 1.9 Cross references: DNA Seq. Acc.: M12674 Insert lengths(kb): 1.899999976158142 Tissue: breast cancer cell line, MCF-7 Gene product: estrogen receptor ESR Alleles: A1, A2 Target Gene: estrogen receptor |
| Insert Size (kb)： | 1.900 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--7.4; ClaI--7.4; HindIII--6.5, 1.3; XbaI--6.4, 1.4; BamHI/EcoRV--7.4; SacI--5.4, 2.1. Electrophoretic analysis of restriction digests suggests that the EcoRV site has been lost. Restriction digests of the clone give the following sizes (kb): HindIII--6.1, 1.3; XbaI--6.0, 1.4. Contains the wild type complete coding sequence. Corresponds to GenBank accession M12674 with a T->G substitution at nt 1491. There are additional SacI sites in the insert. Constructed by inserting an 1.9 kb EcoRI fragment from HEGO into pBluescript SK and then transferring the EcoRV/BamHI fragment to p636. The insert therefore contains some contains some Bluescript sequence. The order of the major features in this plasmid is: ClaI - CMV promoter - SacI - EcoRV - ESR - BamHI - SV40 polyadenylation - HSV-1 tk polyadenylation - |
| References： | Tora L, et al. The cloned human oestrogen receptor contains a mutation which alters its hormone binding properties. EMBO J. 8: 1981-1986, 1989. PubMed: 2792078 Thomas C Spelsberg, personal communication |