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| 产品名称： | KS+SV0CAT |
|---|---|
| 商品货号： | TS160931 |
| Designations： | KS+SV0CAT |
| Depositors： | PA Tsonis |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.9000000953674320 Vector: KS+SV0CAT (plasmid) Promoters: Promoter T3 Construction: cml from pSVOcat, pBluescript KS+ Marker(s):ampR Construct size (kb): 4.900000095367432 Features: insert detection: CAT marker(s): ampR promoter: T3, T7 replicon: pMB1 |
| Applications： | expression vector promoter-cloning vector shuttle vector vector permitting RNA synthesis in vitro |
| Comments： | HindIII-ApaI fragment from pSV2cat contains internal BamHI and EcoRI sites. Restriction digests of the clone give the following sizes (kb): SacI--4.4; Xba--4.4; BamHI--2.9, 1.6; SmaI--4.4; PstI--4.4; EcoRI--3.9, 0.32; HindIII--4.4. Mammalian shuttle expression vector for promoter cloning with activity analysis by CAT (chloramphenicol acetyltransferase) assay. Use KS+SV2CAT (ATCC 37609) as a control. Constructed by inserting the HindIII-ApaI fragment of pSV2cat in HindIII-ApaI digested Bluescript KS+. Contains no SV40 promoter. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Tsonis PA, et al. CAT constructs with convenient sites for cloning and generating deletions. Nucleic Acids Res. 16: 7745, 1988. PubMed: 3166134 Panagiotis A Tsonis, personal communication |
| Shipped： | freeze-dried |