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| 产品名称： | pUXV2 |
|---|---|
| 商品货号： | TS160966 |
| Designations： | pUXV2 |
| Depositors： | JA Bruenn |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.5999999046325680 Vector: pUXV2 (plasmid) Promoters: Promoter gap Construction: pUC19, pCM54, p23 Marker(s):ampR,hygR Construct size (kb): 6.599999904632568 Features: marker(s): ampR, hygR promoter: gap replicon: pMB1, UARS |
| Applications： | expression vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.6, 1.5, 0.33; SacI--6.4; HindIII--3.5, 2.95. Available for research purposes only. One of a series of vectors (ATCC 77463-77465 and 77468-77471) differing only in the selectable marker and the orientation of the gap promoter. Differs from pUXV1 (ATCC 77463) only in the orientation of the gap promoter and the available cloning site. Shuttle expression vector using Ustilago maydis promoters gap (glyceraldehyde phosphate dehydrogenase) and hsp70 to drive expression of the cloned insert and hygromycin B resistance genes respectively. The order of the major features in the plasmid is: Ustilago maydis ARS - ampR - pMB1 ori - 5 lacZ fragment - EcoRI - SacI XhoII - hygR - XhoII -> 3 lacZ fragment. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Kinal H, et al. A family of Ustilago maydis expression vectors: new selectable markers and promoters. Gene 127: 151-152, 1993. PubMed: 8486284 Kinal H, et al. An expression vector for the phytopathogenic fungus, Ustilago maydis. Gene 98: 129-134, 1991. PubMed: 2013404 |
| Shipped： | freeze-dried |
| Shipping Information： | Freeze dried E. coli containing the plasmid. |