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| 产品名称： | C303/L2-T1 C303/L2/T1, C303/L2-T1 |
|---|---|
| 商品货号： | TS160977 |
| Designations： | C303/L2-T1 C303/L2/T1, C303/L2-T1 |
| GenBank Number： | J02691 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | A Tzagoloff |
| Vector： | Construct size (kb): 18.70000076293945 |
| Insert： | DNA: genomic Insert lengths(kb): 8.0 Gene product: phenylalanyl-tRNA synthetase, alpha subunit |
| Insert Size (kb)： | 8.0 |
| Genotype： | MATalpha leu2-3 leu2-112 msf1-1 |
| Mating Type： | alpha |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains the following sites separated by (kb) (approximate): SphI- 1.4- SacI- 0.6- HindIII- 1.6- KpnI- 0.6- HindIII- 0.8- SphI- 1.2- SphI. Restriction digests of the clone give the following sizes (kb): EcoRI/SalI--3.75, 3.5, 3.3, 2.9, 2.3, 2.0, 0.94; XhoI--18.7; XbaI--14.6, 4.2; XbaI/AvaI--4.4, 3.5, 3.1, 2.3, 2.0, 1.8, 1.5; BamHI--18.7. The smallest subclone capable of complementing msf1-1, msf1-3, and msf1-4 mutations contained a 2.2 kb SacI/KpnI fragment. MSF1 is a nuclear gene encoding the alpha subunit of the mitochondrial phenylalanyl-tRNA synthetase. The probe, pG120/T1, has been deposited in Escherichia coli RR1 as TS160977 and in Saccharomyces cerevisiae C303/L2 as ATCC 66317. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Koerner TJ, et al. Isolation and characterization of the yeast gene coding for the alpha subunit of mitochondrial phenylalanyl-tRNA synthetase. J. Biol. Chem. 262: 3690-3696, 1987. PubMed: 3029120 |