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微信小章| 产品名称： | DQ H2.2 |
|---|---|
| 商品货号： | TS160980 |
| Designations： | DQ H2.2 |
| GenBank Number： | M15557 |
| Species： | Oryctolagus cuniculus, rabbit |
| Depositors： | C Leguern, TJ Kindt |
| Applications： | The recommended probe is the 0.5 AccI/PstI fragment corresponding to nt 1166 - 1684 of the reported sequence, which begins toward the 3 end of exon 3 and extends into intron 4 (encoding through the cytoplasmic tail). |
| Vector： | Construct size (kb): 5.0 |
| Insert： | DNA: genomic Insert lengths(kb): 2.200000047683716 Tissue: T-lymphocyte RL-5 cell line Gene product: major histocompatibility complex, class II, DQ alpha 1 RLA-DQA |
| Insert Size (kb)： | 2.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): AccI/PstI--3.2, 0.85, 0.6, 0.3; PstI--3.8, 1.25; EcoRI--3.0, 2.0. The recommended probe is the 0.5 AccI/PstI fragment corresponding to nt 1166 - 1684 of the reported sequence, which begins toward the 3 end of exon 3 and extends into intron 4 (encoding through the cytoplasmic tail). The complete insert begins upstream of exon 2 and continues beyond the last exon (exon 5). Rabbit genomic PvuII fragments detected by this probe correspond to these haplotypes (kb): D1--8.0; D2--1.8; D3--5.9; D4--3; D6--6.3; D7--4.7; D8--6.3; D15--3.0; D17--8.0. Rabbit genomic EcoRI fragments detected by this probe correspond to these haplotypes (kb): D1--2.8; D2--6.3; D3--3.7; D4--9.6; D6--2.8; D7--6.3; D8--6.3; D15--3.3; D17--3.7. |
| References： | Marche PN, et al. Definition of rabbit class I and class II MHC gene haplotypes using molecular typing procedures. Immunogenetics 29: 273-276, 1989. PubMed: 2564845 LeGuern C, et al. Molecular evidence for five distinct MHC class II alpha genes in the rabbit. Immunogenetics 22: 141-148, 1985. PubMed: 3861576 LeGuern C, et al. Sequence determination of a transcribed rabbit class II gene with homology to HLA-DQalpha. Immunogenetics 25: 104-109, 1987. PubMed: 3493213 Thomas J Kindt, personal communication |