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| 产品名称： | pAM401 |
|---|---|
| 商品货号： | TS161004 |
| Designations： | pAM401 |
| Depositors： | DB Clewell |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 10.4 Vector: pAM401 (plasmid) Construction: pACYC184, pGB354 Marker(s):cmlR,tetR Construct size (kb): 10.4 Features: marker(s): cmlR, tetR replicon: p15A, pGB354 |
| Applications： | shuttle vector |
| Comments： | Please note there is an error in the map in the J. Bact. 165: 831-836, 1986 reference. The BclI and EcoRI sites should be reversed. Shuttle vector for Escherichia coli/Streptococcus faecalis. pACYC184 and pGB354 joined at AvaI and SphI sites. |
| Media： | ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Wirth R, et al. Highly efficient protoplast transformation system for streptococcus faecalis and a new Escherichia coli-S. faecalis shuttle vector. J. Bacteriol. 165: 831-836, 1986. PubMed: 3005240 Don B Clewell, personal communication |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |