宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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pUNG15

货号 TS161059
中文名称 null
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产品简介
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产品名称: pUNG15
商品货号: TS161059
Designations: pUNG15
GenBank Number:

X15653

Species: Homo sapiens, human
Vector:
Construct size (kb): 5.073999881744385
Insert:
DNA: cDNA
Insert lengths(kb): 2.099999904632568
Tissue: placenta
Gene product: uracil-DNA glycosylase(uracil DNA glycosylase) DGU
Insert Size (kb): 2.100
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): XbaI/SalI--3.0, 2.1; BamHI--3.3, 1.5, 0.3; HindIII--4.2, 0.9; EcoRI--3.0, 2.1; XhoI--5.1.
The insert contains the following restriction sites (nt positions according to the published sequence): BamHI--297, 538; SacII--315; StuI--590; NcoI--670; HindIII--1132.
Contains the complete sequenced cDNA, with the coding region extending from nt 107 to nt 1021. The poly(A) tail of 17 bp is 16 bp downstream of the canonical polyadenylation signal.
Detects a mRNA of 2.4 kb in placenta.
Oriented such that transcription from the T7 promoter gives the sense strand.
References:

Olsen LC, et al. Molecular cloning of human uracil-DNA glycosylase, a highly conserved DNA repair enzyme*. EMBO J. 8: 3121-3125, 1989. PubMed: 2555154

Aasland R, et al. Chromosomal assignment of human uracil-DNA glycosylase to chromosome 12. Genomics 7: 139-141, 1990. PubMed: 2335354

Lisbeth C Olsen, personal communication