| 产品名称: | pUNG15 |
|---|---|
| 商品货号: | TS161059 |
| Designations: | pUNG15 |
| GenBank Number: | X15653 |
| Species: | Homo sapiens, human |
| Vector: | Construct size (kb): 5.073999881744385 |
| Insert: | DNA: cDNA Insert lengths(kb): 2.099999904632568 Tissue: placenta Gene product: uracil-DNA glycosylase(uracil DNA glycosylase) DGU |
| Insert Size (kb): | 2.100 |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments: | Restriction digests of the clone give the following sizes (kb): XbaI/SalI--3.0, 2.1; BamHI--3.3, 1.5, 0.3; HindIII--4.2, 0.9; EcoRI--3.0, 2.1; XhoI--5.1. The insert contains the following restriction sites (nt positions according to the published sequence): BamHI--297, 538; SacII--315; StuI--590; NcoI--670; HindIII--1132. Contains the complete sequenced cDNA, with the coding region extending from nt 107 to nt 1021. The poly(A) tail of 17 bp is 16 bp downstream of the canonical polyadenylation signal. Detects a mRNA of 2.4 kb in placenta. Oriented such that transcription from the T7 promoter gives the sense strand. |
| References: | Olsen LC, et al. Molecular cloning of human uracil-DNA glycosylase, a highly conserved DNA repair enzyme*. EMBO J. 8: 3121-3125, 1989. PubMed: 2555154 Aasland R, et al. Chromosomal assignment of human uracil-DNA glycosylase to chromosome 12. Genomics 7: 139-141, 1990. PubMed: 2335354 Lisbeth C Olsen, personal communication |