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| 产品名称： | pUC-DIS |
|---|---|
| 商品货号： | TS161126 |
| Designations： | pUC-DIS |
| Depositors： | NC Barbet, AM Carr |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 2.7000000476837160 Vector: pUC-DIS (plasmid) Promoters: Promoter lac Construction: pUC18 Marker(s):ampR Construct size (kb): 2.700000047683716 Features: insert detection: lacZ marker(s): ampR promoter: lac replicon: pMB1 |
| Applications： | vector for other uses vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): SphI--2.7; SacI--2.7; KpnI--2.7; SalI--2.7. Vector with a MCS containing only relatively rare enzyme cutting sites flanked by SfiI sites to facilitate removal of the insert. By reducing the number of sites in the plasmid, more sites in an insert can be used for gene disruption experiments. The order of the major features in this plasmid is: SfiI/SacI/MCS/SfiI/lacZ - pMB1 ori - ampR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Barbet N, et al. Versatile shuttle vectors and genomic libraries for use with Schizosaccharomyces pombe. Gene 114: 59-66, 1992. PubMed: 1587485 |
| Shipped： | freeze-dried |
| Shipping Information： | xa0Freeze dried xa0Escherichia coli containing the plasmid. |