宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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pAE Bam-RI [probe F]

货号 TS161149
中文名称 null
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产品名称: pAE Bam-RI probe F
商品货号: TS161149
Designations: pAE Bam-RI probe F
Species: avian erythroblastosis virus (type C)
Depositors: J Bishop
Vector:
Construct size (kb): 4.5
Insert:
DNA: genomic
Insert lengths(kb): 0.5
Gene product: epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene homolog) v-erb-b
Insert Size (kb): 0.5
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments:
Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--3.9, 0.6; BamHI--4.6; EcoRI--4.6; PvuII--2.45, 2.2.
The insert is the erbB-specific BamHI-EcoRI fragment of the AEV genome.
References:

Vennstrom B, et al. Molecular cloning of the avian erythroblastosis virus genome and recovery of oncogenic virus by transfection of chicken cells. J. Virol. 36: 575-585, 1980. PubMed: 6253678

Vennstrom B, Bishop JM. Isolation and characterization of chicken DNA homologous to the two putative oncogenes of avian erythroblastosis virus. Cell 28: 135-143, 1982. PubMed: 6279309